When calling variants on RNA-seq, allele frequencies can, although e.g. investigating a diploid sample, take any value from 0.0 to 1.0 again, because we don't know which allele is expressed in what amount.
This situation can be easily modeled in Varlociraptor.
RNA-seq variant calling can however suffer from other issues like splicing induced artifacts. The right way of mapping and processing reads for RNA-seq variant calling is still an area of active research, and we suggest to carefully investigate the current state of the art preprocessing steps, and possible solutions to avoid such artifacts (post-mapping cleanup, mapping against transcriptome, assembly-first, ...).
samples:
hamletsrna:
universe: "[0.0,1.0]"
events:
present: "hamletsrna:]0.0,1.0]"