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use std::ptr;
use std::slice;
use std::ffi;
use std::i32;
use std::f32;
use std::fmt;
use ieee754::Ieee754;
use itertools::Itertools;
use htslib;
const MISSING_INTEGER: i32 = i32::MIN;
const VECTOR_END_INTEGER: i32 = i32::MIN + 1;
lazy_static!{
static ref MISSING_FLOAT: f32 = f32::from_bits(0x7F800001);
static ref VECTOR_END_FLOAT: f32 = f32::from_bits(0x7F800002);
}
pub trait Numeric {
fn is_missing(&self) -> bool;
fn missing() -> Self;
}
impl Numeric for f32 {
fn is_missing(&self) -> bool {
self.bits() == MISSING_FLOAT.bits()
}
fn missing() -> f32 {
*MISSING_FLOAT
}
}
impl Numeric for i32 {
fn is_missing(&self) -> bool {
*self == MISSING_INTEGER
}
fn missing() -> i32 {
MISSING_INTEGER
}
}
trait NumericUtils {
fn is_vector_end(&self) -> bool;
}
impl NumericUtils for f32 {
fn is_vector_end(&self) -> bool {
self.bits() == VECTOR_END_FLOAT.bits()
}
}
impl NumericUtils for i32 {
fn is_vector_end(&self) -> bool {
*self == VECTOR_END_INTEGER
}
}
pub struct Record {
pub inner: *mut htslib::vcf::bcf1_t,
pub header: *mut htslib::vcf::bcf_hdr_t,
buffer: *mut ::libc::c_void,
}
impl Record {
pub fn new() -> Self {
let inner = unsafe { htslib::vcf::bcf_init() };
Record { inner: inner, header: ptr::null_mut(), buffer: ptr::null_mut() }
}
pub fn inner(&self) -> &htslib::vcf::bcf1_t {
unsafe { &*self.inner }
}
pub fn inner_mut(&mut self) -> &mut htslib::vcf::bcf1_t {
unsafe { &mut *self.inner }
}
pub fn rid(&self) -> Option<u32> {
match self.inner().rid {
-1 => None,
rid => Some(rid as u32)
}
}
pub fn pos(&self) -> u32 {
self.inner().pos as u32
}
pub fn set_pos(&mut self, pos: i32) {
self.inner_mut().pos = pos;
}
pub fn alleles(&self) -> Vec<&[u8]> {
unsafe { htslib::vcf::bcf_unpack(self.inner, htslib::vcf::BCF_UN_STR) };
let n = self.inner().n_allele as usize;
let dec = self.inner().d;
let alleles = unsafe { slice::from_raw_parts(dec.allele, n) };
(0..n).map(|i| unsafe { ffi::CStr::from_ptr(alleles[i]).to_bytes() }).collect()
}
pub fn qual(&self) -> f32 {
self.inner().qual
}
pub fn set_qual(&mut self, qual: f32) {
self.inner_mut().qual = qual;
}
pub fn info<'a>(&'a mut self, tag: &'a [u8]) -> Info {
Info { record: self, tag: tag }
}
pub fn sample_count(&self) -> u32 {
self.inner().n_fmt_n_sample >> 8
}
pub fn allele_count(&self) -> u16 {
self.inner().n_allele
}
pub fn genotypes(&mut self) -> Result<Genotypes, FormatReadError> {
Ok(Genotypes {
encoded: try!(self.format(b"GT").integer())
})
}
pub fn format<'a>(&'a mut self, tag: &'a [u8]) -> Format {
Format::new(self, tag)
}
pub fn push_format_integer(&mut self, tag: &[u8], data: &[i32]) -> Result<(), TagWriteError> {
self.push_format(tag, data, htslib::vcf::BCF_HT_INT)
}
pub fn push_format_float(&mut self, tag: &[u8], data: &[f32]) -> Result<(), TagWriteError> {
self.push_format(tag, data, htslib::vcf::BCF_HT_REAL)
}
fn push_format<T>(&mut self, tag: &[u8], data: &[T], ht: i32) -> Result<(), TagWriteError> {
assert!(data.len() > 0);
unsafe {
if htslib::vcf::bcf_update_format(
self.header,
self.inner,
ffi::CString::new(tag).unwrap().as_ptr() as *mut i8,
data.as_ptr() as *const ::libc::c_void,
data.len() as i32,
ht
) == 0 {
Ok(())
}
else {
Err(TagWriteError::Some)
}
}
}
pub fn push_info_integer(&mut self, tag: &[u8], data: &[i32]) -> Result<(), TagWriteError> {
self.push_info(tag, data, htslib::vcf::BCF_HT_INT)
}
pub fn push_info_float(&mut self, tag: &[u8], data: &[f32]) -> Result<(), TagWriteError> {
self.push_info(tag, data, htslib::vcf::BCF_HT_REAL)
}
pub fn push_info<T>(&mut self, tag: &[u8], data: &[T], ht: i32) -> Result<(), TagWriteError> {
assert!(data.len() > 0);
unsafe {
if htslib::vcf::bcf_update_info(
self.header,
self.inner,
ffi::CString::new(tag).unwrap().as_ptr() as *mut i8,
data.as_ptr() as *const ::libc::c_void,
data.len() as i32,
ht
) == 0 {
Ok(())
}
else {
Err(TagWriteError::Some)
}
}
}
pub fn trim_alleles(&mut self) -> Result<(), TrimAllelesError> {
match unsafe { htslib::vcfutils::bcf_trim_alleles(self.header, self.inner) } {
-1 => Err(TrimAllelesError::Some),
_ => Ok(())
}
}
}
#[derive(Debug)]
pub enum GenotypeAllele {
Unphased(i32),
Phased(i32),
UnphasedMissing,
PhasedMissing
}
impl GenotypeAllele {
pub fn from_encoded(encoded: i32) -> Self {
match (encoded, encoded & 1) {
(0, 0) => GenotypeAllele::UnphasedMissing,
(1, 1) => GenotypeAllele::PhasedMissing,
(e, 1) => GenotypeAllele::Phased((e >> 1) - 1),
(e, 0) => GenotypeAllele::Unphased((e >> 1) - 1),
_ => panic!("unexpected phasing type")
}
}
pub fn index(&self) -> Option<u32> {
match self {
&GenotypeAllele::Unphased(i) => Some(i as u32),
&GenotypeAllele::Phased(i) => Some(i as u32),
&GenotypeAllele::UnphasedMissing => None,
&GenotypeAllele::PhasedMissing => None
}
}
}
impl fmt::Display for GenotypeAllele {
fn fmt(&self, f: &mut fmt::Formatter) -> fmt::Result {
match self.index() {
Some(a) => write!(f, "{}", a),
None => write!(f, ".")
}
}
}
custom_derive! {
#[derive(NewtypeDeref, Debug)]
pub struct Genotype(Vec<GenotypeAllele>);
}
impl fmt::Display for Genotype {
fn fmt(&self, f: &mut fmt::Formatter) -> fmt::Result {
let &Genotype(ref alleles) = self;
try!(write!(f, "{}", alleles[0]));
for a in &alleles[1..] {
let sep = match a {
&GenotypeAllele::Phased(_) => '|',
&GenotypeAllele::Unphased(_) => '/',
&GenotypeAllele::UnphasedMissing => '/',
&GenotypeAllele::PhasedMissing => '|'
};
try!(write!(f, "{}{}", sep, a));
}
Ok(())
}
}
#[derive(Debug)]
pub struct Genotypes<'a> {
encoded: Vec<&'a [i32]>
}
impl<'a> Genotypes<'a> {
pub fn get(&self, i: usize) -> Genotype {
let igt = self.encoded[i];
let gt = Genotype(igt.into_iter().map(|&e| GenotypeAllele::from_encoded(e)).collect_vec());
gt
}
}
impl Drop for Record {
fn drop(&mut self) {
if !self.buffer.is_null() {
unsafe { ::libc::free(self.buffer) };
}
unsafe { htslib::vcf::bcf_destroy(self.inner) };
}
}
unsafe impl Send for Record {}
unsafe impl Sync for Record {}
pub struct Info<'a> {
record: &'a mut Record,
tag: &'a [u8],
}
impl<'a> Info<'a> {
fn data(&mut self, data_type: i32) -> Result<Option<(usize, i32)>, InfoReadError> {
let mut n: i32 = 0;
match unsafe {
htslib::vcf::bcf_get_info_values(
self.record.header,
self.record.inner,
ffi::CString::new(self.tag).unwrap().as_ptr() as *mut i8,
&mut self.record.buffer,
&mut n,
data_type
)
} {
-1 => Err(InfoReadError::UndefinedTag),
-2 => Err(InfoReadError::UnexpectedType),
-3 => Ok(None),
ret => Ok(Some((n as usize, ret))),
}
}
pub fn integer(&mut self) -> Result<Option<&'a [i32]>, InfoReadError> {
self.data(htslib::vcf::BCF_HT_INT).map(|data| data.map(|(n, _)| {
trim_slice(
unsafe { slice::from_raw_parts(self.record.buffer as *const i32, n) }
)
}))
}
pub fn integer_mut(&mut self) -> Result<Option<&'a mut [i32]>, InfoReadError> {
self.data(htslib::vcf::BCF_HT_INT).map(|data| data.map(|(n, _)| {
unsafe { slice::from_raw_parts_mut(self.record.buffer as *mut i32, n) }
}))
}
pub fn float(&mut self) -> Result<Option<&'a [f32]>, InfoReadError> {
self.data(htslib::vcf::BCF_HT_REAL).map(|data| data.map(|(n, _)| {
trim_slice(
unsafe { slice::from_raw_parts(self.record.buffer as *const f32, n) }
)
}))
}
pub fn float_mut(&mut self) -> Result<Option<&'a mut [f32]>, InfoReadError> {
self.data(htslib::vcf::BCF_HT_REAL).map(|data| data.map(|(n, _)| {
unsafe { slice::from_raw_parts_mut(self.record.buffer as *mut f32, n) }
}))
}
pub fn flag(&mut self) -> Result<bool, InfoReadError> {
self.data(htslib::vcf::BCF_HT_FLAG).map(|data| {
match data {
Some((_, ret)) => ret == 1,
None => false
}
})
}
pub fn string(&mut self) -> Result<Option<Vec<&'a [u8]>>, InfoReadError> {
self.data(htslib::vcf::BCF_HT_STR).map(|data| data.map(|(n, ret)| {
unsafe {
slice::from_raw_parts(self.record.buffer as *const u8, ret as usize)
}.chunks(n).map(|s| {
s.split(|c| *c == 0u8).next().expect("Bug: returned string should not be empty.")
}).collect()
}))
}
pub fn string_mut(&mut self) -> Result<Option<Vec<&'a mut [u8]>>, InfoReadError> {
self.data(htslib::vcf::BCF_HT_STR).map(|data| data.map(|(n, ret)| {
unsafe {
slice::from_raw_parts_mut(self.record.buffer as *mut u8, ret as usize)
}.chunks_mut(n).collect()
}))
}
}
unsafe impl<'a> Send for Info<'a> {}
unsafe impl<'a> Sync for Info<'a> {}
fn trim_slice<T: PartialEq + NumericUtils>(s: &[T]) -> &[T] {
s.split(|v| v.is_vector_end()).next().expect("Bug: returned slice should not be empty.")
}
pub struct Format<'a> {
record: &'a mut Record,
tag: &'a [u8],
inner: *mut htslib::vcf::bcf_fmt_t,
}
impl<'a> Format<'a> {
fn new(record: &'a mut Record, tag: &'a [u8]) -> Format<'a> {
let inner = unsafe { htslib::vcf::bcf_get_fmt(
record.header,
record.inner,
ffi::CString::new(tag).unwrap().as_ptr() as *mut i8
) };
Format { record: record, tag: tag, inner: inner }
}
pub fn inner(&self) -> &htslib::vcf::bcf_fmt_t {
unsafe { &*self.inner }
}
pub fn inner_mut(&mut self) -> &mut htslib::vcf::bcf_fmt_t {
unsafe { &mut *self.inner }
}
fn values_per_sample(&self) -> usize {
self.inner().n as usize
}
fn data(&mut self, data_type: i32) -> Result<(usize, i32), FormatReadError> {
let mut n: i32 = 0;
match unsafe {
htslib::vcf::bcf_get_format_values(
self.record.header,
self.record.inner,
ffi::CString::new(self.tag).unwrap().as_ptr() as *mut i8,
&mut self.record.buffer,
&mut n,
data_type
)
} {
-1 => Err(FormatReadError::UndefinedTag),
-2 => Err(FormatReadError::UnexpectedType),
-3 => Err(FormatReadError::MissingTag),
ret => Ok((n as usize, ret)),
}
}
pub fn integer(&mut self) -> Result<Vec<&'a [i32]>, FormatReadError> {
self.data(htslib::vcf::BCF_HT_INT).map(|(n, _)| {
unsafe {
slice::from_raw_parts(self.record.buffer as *const i32, n)
}.chunks(self.values_per_sample()).map(|s| trim_slice(s)).collect()
})
}
pub fn integer_mut(&mut self) -> Result<Vec<&'a mut [i32]>, FormatReadError> {
self.data(htslib::vcf::BCF_HT_INT).map(|(n, _)| {
unsafe {
slice::from_raw_parts_mut(self.record.buffer as *mut i32, n)
}.chunks_mut(self.values_per_sample()).collect()
})
}
pub fn float(&mut self) -> Result<Vec<&'a [f32]>, FormatReadError> {
self.data(htslib::vcf::BCF_HT_REAL).map(|(n, _)| {
unsafe {
slice::from_raw_parts(self.record.buffer as *const f32, n)
}.chunks(self.values_per_sample()).map(|s| trim_slice(s)).collect()
})
}
pub fn float_mut(&mut self) -> Result<Vec<&'a mut [f32]>, FormatReadError> {
self.data(htslib::vcf::BCF_HT_REAL).map(|(n, _)| {
unsafe {
slice::from_raw_parts_mut(self.record.buffer as *mut f32, n)
}.chunks_mut(self.values_per_sample()).collect()
})
}
pub fn string(&mut self) -> Result<Vec<&'a [u8]>, FormatReadError> {
self.data(htslib::vcf::BCF_HT_STR).map(|(n, _)| {
unsafe {
slice::from_raw_parts(self.record.buffer as *const u8, n)
}.chunks(self.values_per_sample()).map(|s| {
s.split(|c| *c == 0u8).next().expect("Bug: returned string should not be empty.")
}).collect()
})
}
pub fn string_mut(&mut self) -> Result<Vec<&'a mut [u8]>, FormatReadError> {
self.data(htslib::vcf::BCF_HT_STR).map(|(n, _)| {
unsafe {
slice::from_raw_parts_mut(self.record.buffer as *mut u8, n)
}.chunks_mut(self.values_per_sample()).collect()
})
}
}
unsafe impl<'a> Send for Format<'a> {}
unsafe impl<'a> Sync for Format<'a> {}
quick_error! {
#[derive(Debug)]
pub enum InfoReadError {
UndefinedTag {
description("tag undefined in header")
}
UnexpectedType {
description("tag type differs from header definition")
}
}
}
quick_error! {
#[derive(Debug)]
pub enum FormatReadError {
UndefinedTag {
description("tag undefined in header")
}
UnexpectedType {
description("tag type differs from header definition")
}
MissingTag {
description("tag missing from record")
}
}
}
quick_error! {
#[derive(Debug)]
pub enum TagWriteError {
Some {
description("error writing tag to record")
}
}
}
quick_error! {
#[derive(Debug)]
pub enum TrimAllelesError {
Some {
description("error trimming alleles")
}
}
}